The 23-year-old All Star suffered an unspecified lower-body injury Thursday in the Blue Jackets' win over Dallas and is expected to miss at least a week. Werenski limped off the ice and headed for the locker room late in the game.
Paired with Seth Jones on the top defensive line, Werenski has a goal and three assists in 12 games.
Columbus recalled forward Liam Foudy from the taxi squad to take Werenski's spot on the roster. The Blue Jackets open a back-to-back with Carolina on Sunday afternoon.