Barca broke the attendance record for a women's game in the previous round when 91,553 fans turned out to see them beat Real Madrid 5-2 last Wednesday.
Camp Nou officially holds 99,354, though, so they could break their own record when they host Wolfsburg on April 22.
While the quarterfinal was also sold out, not everyone with a ticket turned up due to the early kickoff -- tickets went on sale before the time was announced -- and the wet weather on the day of the game.
The second leg of the Wolfsburg tie will be played at the Volkswagen Arena on April 30, with the winners facing either Lyon or Paris Saint-Germain in the final in Turin on May 21.
Barca are the current holders of the competition having won it for the first time last season as part of a Treble, which also saw them win the Primera Division and the Copa de la Reina.
They could yet end this season with four trophies. They have already won the Spanish Super Copa and the league and remain in the hunt for the Champions League and the Copa.
Wolfsburg, meanwhile, are top of the Frauen-Bundesliga, four points clear of Bayern Munich, who they beat 6-0 at the weekend.
Their tie with Barca is a repeat of the 2020 semifinal, which Wolfsburg won 1-0 before losing the final to Lyon.
Barca's success since then has taken interest in the club's women's team to a new level. Last season's Treble, as well as individual recognition for captain Alexia Putellas, the holder of the Ballon d'Or and FIFA's The Best, saw that interest translate into ticket sales for the game against Madrid last week.
That was just the second competitive game they had played at Camp Nou and the first with fans. The previous time they played there was behind closed doors due to the pandemic.
Tickets for the Wolfsburg game were made available to members for a processing fee on Monday and all 50,000 were snapped up before the end of the day.
They then went on general sale on Tuesday, with prices ranging from €11 to €26, and were sold out on the same day.
The previous record crowd for a women's game had stood since 1999, when 90,185 fans watched the World Cup final between the United States and China at the Rose Bowl in Pasadena.
However, some estimates suggest 110,000 people attended Denmark's win over Mexico at the Azteca Stadium in the 1971 World Cup final, a tournament that was not officially sanctioned.