Newly re-signed Aston Villa striker Christian Benteke could not help his club from falling to a 2-0 defeat to non-league side Luton Town.
Benteke, who recently committed his future to Villa after handing in a transfer request, played the first 45 minutes of the match before being substituted.
Strikers Jake Howells and John Shaw grabbed the goals for Luton Town.
Other Premier League teams in action on Tuesday included West Ham, who picked up their first win of their tour of Germany, beating Hamburg 3-1.
Matt Jarvis proved to be the main man for the East Londoners, setting up goals for both Alou Diarra and James Tomkins before winning a spot kick converted by Ravel Morrison.
The home side briefly halved the deficit through Heiko Westermann.
Premier League new boys Crystal Palace coasted past League 1 Gillingham 3-0 at the Priestfield. Former Exeter youngster Dean Moxey opened the scoring with a crisp drive from 30 yards before Stephen Dobbie made it two shortly after the break. Dobbie then set up trialist Quincy Owusu-Abeyie for the third.
Ten-man Newcastle United drew 1-1 in a bad-tempered game with Pacos de Ferreira. The Portuguese side took an early lead through Caetano, before a double sending-off which saw Caetano and Toon captain Fabricio Coloccini given their marching orders for a first-half clash. Shola Ameobi levelled the match with a powerful finish in the 60th minute.
Hull City are also in Portugal, where they shared a goalless draw with FC Braga.