Harlequins will play the Maori All Blacks at their Twickenham Stoop home on November 16.
The game forms part of Harlequins' 150th year anniversary and will follow the Maori All Blacks' games against the USA Eagles in Chicago and Munster in Ireland.
Harlequins CEO David Ellis said: "In July 2016 Harlequins will become 150 years old and this match against the Maori All Blacks will form an integral part of our year- long celebrations.
"The two sides previously met in 1926 and I'm sure this match will be very popular among our supporters. Like Harlequins, the Maori All Blacks have a proud culture and heritage and we are looking forward to hosting them at The Stoop in November."
Maori All Blacks chairman Wayne Peters added: "The Maori All Blacks are an important part of New Zealand Rugby's high performance strategy - and when they play on the international stage it is an opportunity to press for other honours.
"This is a fantastic tour for players and for fans in the USA, Ireland and UK. We're delighted to be taking the Maori All Blacks on what will be a challenging and exciting campaign.
"We're incredibly proud of this team and their ability to take their culture and style of rugby to the world. They're a special team that is popular anywhere they travel and they're always exciting to watch."