Manchester United defender Axel Tuanzebe, Chelsea's Jake Clarke-Salter and Norwich's James Maddison have all been handed their first England under-21 call-ups.
The trio are in Aidy Boothroyd's squad for their Euro 2019 qualifier in Ukraine on Nov. 10.
They help fill the gap left by Joe Gomez, Ruben Loftus-Cheek and Tammy Abraham after they were selected for Gareth Southgate's senior squad.
Clarke-Salter and Tuanzebe have both played under Boothroyd for the U20s last term and Maddison has been with the Young Lions once before when he was called up for an U20 match with Canada in March 2016, but was uncapped.
The Young Lions go to Ukraine sitting top of Group Four after wins over Scotland and Andorra last month.
With only one game during November, the squad will return home after the game for more training together as Boothroyd looks to build a firm base for his group going into 2018.
Goalkeepers: Angus Gunn (Norwich City on loan from Man City), Dean Henderson (Shrewsbury Town on loan from Man United), Freddie Woodman (Newcastle)
Defenders: Trent Alexander-Arnold (Liverpool), Ben Chilwell (Leicester City), Jake Clarke Salter (Chelsea), Dael Fry (Middlesbrough), Jonjoe Kenny (Everton), Fikayo Tomori (Hull City on loan from Chelsea), Axel Tuanzebe (Manchester United), Kyle Walker-Peters (Tottenham), Joseph Worrall (Nottingham Forest)
Midfielders: Lewis Cook (Bournemouth), Tom Davies (Everton), Kieran Dowell (Nottingham Forest on loan from Everton), Josh Onomah (Aston Villa on loan from Tottenham), Ainsley Maitland-Niles (Arsenal)
Forwards: Dominic Calvert-Lewin (Everton), Demarai Gray (Leicester City), Jack Harrison (New York City FC), Ademola Lookman (Everton), James Maddison (Norwich City), Dominic Solanke (Liverpool)